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Gilgo Beach murders investigators were seen using K-9 dogs to search a Long Island area where a woman's body was found in 1993.
Multiple law enforcement agencies were on the ground in North Sea on Friday, as first reported by News12 Long Island.
Their search has gone into the area were Sandra Costilla's body was found three decades ago in a murder case that remains unsolved.
Convicted serial killer John Bittrolff, who lived near the search area, was one suspected of the murder but never charged.
Bittrolff is serving a 50-year sentence for the murders of two women.
Multiple law enforcement agencies were on the ground in North Sea, Long Island, on Friday
Their search has gone into the area were Sandra Costilla's body was found three decades ago in a murder case that remains unsolved
Convicted serial killer John Bittrolff, who lived near the search area, was one suspected of the murder but never charged. Costilla is pictured above
John Bittrolff is pictured. He is serving a 50-year sentences for the murders of two women
The Suffolk County District Attorney's office did not provide additional information about the search when contacted by DailyMail.com.
'The Suffolk County Police Department, the New York Police Department and the New York State Police are working with the District Attorney's Office on an ongoing investigation,' the DA's statement read.
'We do not comment on investigative steps while they are underway. We will make further statements when appropriate.'
It comes after Suffolk County detectives began searching a wooded area in Manorville, New York on Tuesday, win connection with the Gilgo Beach murders investigation.
One local resident, Mike, told DailyMail.com cops were looking in his backyard.
'I have five acres. I told the guys we know every god damn dirt trail from Manorville to Calverton and then some,' he said.
'There is one pond called Deep Pond that more than 70 feet deep of black water - I’d have divers in the lake I’d tell you that - that is what I would do.'
Rex Heuermann, 60, is charged with murdering four female sex workers whose bodies were found buried on a remote stretch of Gilgo Beach more than 10 years ago.
He was arrested in July and initially charged with killing Melissa Barthelemy, 24, Megan Waterman, 22, and Amber Lynn Costello 27.
A new search is underway in connection to the Gilgo Beach murder investigation, according to police sources. Rex Heuermann, 60, is charged with murdering four female sex workers
Multiple agencies searching Manorville location in connection with Gilgo Beach investigation
Cops with K-9 units searched the Manorville Woods on Wednesday
The women known as the 'Gilgo Four' were discovered close to Heuermann's Long Island home. However, other bodies - including those of sex workers - have been found in the area
The first victim, 24-year-old Melissa Barthelemy, was discovered by Suffolk County Police on December 11, 2010. The body of Megan Waterman, 22, from Maine, was found two days later
Heuermann is also charged with killing Amber Costello (left) and Maureen Brainard-Barnes (right)
DNA found on the burlap wrapped around Waterman's body was a 99.96 percent match to samples from a discarded pizza crust and napkin in a garbage bin outside Heuermann's firm, police said
In February, he was also charged with the murder of Maureen Brainard-Barnes, 25.
The searches come after the father-of-two's home in Massapequa was raided following discovery of the bodies.
The women were found wrapped in burlap and buried along the remote stretch of Ocean Parkway on Long Island's South Shore over a decade ago.
Heuermann has pleaded not guilty to all counts and currently remains in jail awaiting trial.
The women's remains were discovered during the search for 23-year-old Shannan Gilbert, an escort from New Jersey who had vanished in May 2010 after making a frantic 911 call.
Her remains were found along the same stretch of coast in December 2011 - and it was her body that led police to the discovery of the 'Gilgo Four'.
No one has ever been charged in Gilbert's death and police said it may have been accidental - but her family believe she was murdered.
The women were among 11 found on the desolate stretch of coastline close to Heuermann's Long Island Home between 2010 and 2011.
All of the victims worked as escorts who advertised themselves on Craigslist.
Heuermann, a father-of-two, has been held in jail without bail since his arrest and has pleaded not guilty
Police confirmed they are probing Heuermann's potential link to the murders of two other sex workers: Valerie Mack, 25, went missing around the summer of 2000. Her remains were located that September, and more remains were discovered nearly 11 years later
The Heuermann family home in Massapequa Park was torn apart by authorities as they combed the property for evidence
The Suffolk County District Attorney's Office declined to comment on the nature of the new search. Pictured: Investigators suit up outside Heuermann's home last July
Heuermann was linked to the killings by DNA on the burlap used to transport the bodied which was compared to samples taken from a pizza crust and napkin that were discarded outside his Manhattan architectural firm, police said.
The samples boasted a 99.96 percent match, according to law enforcement.
Prior to testing the DNA, cops said they were alerted to Heuermann as a potential suspect after a witness linked Heuermann's Chevrolet Avalanche to Costello's murder.
The car was then tied to Heuermann's cellphone records, which allegedly linked him to locations related to the murders.
The indictment stated that Heuermann had used different burner phones to contact each of his victims.
Cops also accused Heuermann of using Barthelemy's phone to make taunting phone calls to her family from the comfort of his office.
Her sister Melissa told how she was bombarded with threatening phone calls from the killer who boasted of the killing and was keeping tabs on her movements.
In October, it was revealed that Heuermann is being probed over the slaying of two additional sex workers.
Suffolk County Police Commissioner Rodney K. Harrison confirmed he had assigned additional investigators to the case of two other women, Valerie Mack and Karen Vergata.
Alleged Gilgo Beach serial killer Rex A. Heuermann, right, appears in Judge Tim Mazzei's courtroom next to his attorney Michael Brown at Suffolk County Court in Riverhead on Apri l17
His ex-wife, Asa Ellerup (pictured) was also spotted pulling up in a black Mercedes outside the Long Island courthouse
James Burke, the disgraced former head of Suffolk County Police Department, has been accused of hampering the Gilgo Beach serial killer probe by booting the FBI off the case in its early stages
At the suspected time of the murders, Heuermann's now ex-wife Asa Ellerup was away from home. She filed for divorce less than a week after he husband's arrest.
The accused killer was back in court for a procedural hearing on April 17.
He listened intently to the hearing, which centered around troves of potential evidence seized from various devices related to the case.
Ellerup was also spotted pulling up in a black Mercedes outside the Long Island courthouse.
Heuermann's lawyer is now attempting to shift the blame for the Gilgo Beach murders on to the corrupt former police chief who was previously in charge of the case.
Speaking outside the Long Island courthouse where his accused serial killer client had a hearing last week, attorney Michael Brown said disgraced PC James Burke is 'involved' in the sordid affair.
'We received approximately almost 3,000 leads on this case,' he told reporters outside Suffolk County Supreme Court in Riverhead.
He appeared in court in September accused of several offences including assault, public lewdness and unlawful solicitation after flashing in a park
Exclusive DailyMail.com photos showed disgraced Suffolk police chief James Burke stepping out in public for the first time since a suspect was arrested in the Gilgo Beach murders case
Burke pictured in 2015 outside an FBI office in New York after he was arrested and charged for beating a thief who took a bag of porn and sex toys from his police car then trying to cover up the attack
'There were numerous leads that came into the Suffolk County Police Department, suggesting that Chief Burke was involved in this.'
Burke allegedly led a double life involving crack, cross-dressing and relationships with prostitutes.
He pleaded not guilty several offences including assault, public lewdness and unlawful solicitation after flashing in a park during a court appearance in September last year.
Burke was previously convicted and jailed on federal charges for beating a handcuffed man suspected of stealing pornography, sex toys and other items from his department SUV.
The scandal also toppled the county's longtime district attorney and the county's top corruption prosecutor, who were convicted of helping Burke cover up the beating.