Jeopardy! Masters contestant James Holzhauer leaves viewers in stitches with a VERY cheeky spelling quip: 'Gotta be careful with that fourth letter!'

Jeopardy! Masters contestant James Holzhauer couldn't resist making a cheeky quip about the word 'fuchsia,' leaving the audience in hysterics as he highlighted the risk that comes with spelling out the word during Wednesday night's season premiere.

The 39-year-old quizzer, who is among the returning players competing in the 18-game competition, made the joke as he took on the Spell It category, which sees competitors tasked with spelling their answers aloud.

It all started when host Ken Jennings, 49, asked: 'The last name of botanist Leonhard gave us this word for a type of flower and shade of reddish purple.'

James then buzzed in and successfully spelled out the word 'fuchsia.'

Jeopardy! Masters contestant James Holzhauer had the audience in fits of laughter during Wednesday night's season premiere

Hilarity ensued after host Ken Jennings asked: 'The last name of botanist Leonhard gave us this word for a type of flower and shade of reddish purple'

James then quipped: 'Gotta be careful with that fourth letter there.'

The quip tickled the audience who could be heard laughing in the studio.

Fans at home were also in stitches over the comments and shared their amusement on social media.

One wrote: 'I laughed that one I can't stop what James said. Haha!!'

Agreeing, another said: 'Same laughed TOO hard.'

A third added: 'I loved watching James!'

A fourth said: 'Tonight's show was hilarious.'

James's cheeky spelling gag wasn't his only funny moment, as he also described himself as the 'main event' during a chat with Ken.

James, 39,  playfully quipped that he had to 'be careful with that fourth letter there' on spelling 'fuchsia'

James is among those competing in the Jeopardy! Masters tournament and is best known for his 32-game winning streak as Jeopardy! champion

Ken asked: 'You feel like to win Jeopardy! Masters it's going to have to go through you?'

James then entertained the audience when he replied: 'That is how a final boss works, Ken.'

James is best known for his 32-game winning streak as Jeopardy! champion from April to June 2019, during which time he set multiple single-game records for winnings, and for winning the following Tournament of Champions that November.

He won an impressive $2,464,216 in his 33 appearances, making him the second-highest winner on the show in regular-play non-tournament winnings behind only Ken, who won $2,520,700 in 2004.

Jeopardy! Masters returned on May 1 with host Ken Jennings. The new series will conclude on May 22

Professional sports gambler James returned for Jeopardy! Masters alongside Matt Amodio, Mattea Roach, Victoria Groce, Yogesh Raut, and Amy Schneider.

The tournament kicked off on May 1 and is scheduled to conclude with a grand final on May 22.

Each episode will consist of two half-hour games, and the winner of each is awarded three points, while the runner-up receives one.

The four players with the most points will then advance to the semifinals, and the top three competitors will face off in finals for the $500,000 grand prize and Trebek Trophy.

Jeopardy! Masters continues on Monday, May 6 (8 EDT/PDT) and airs Mondays, Wednesdays and Fridays