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Princess Charlotte appeared to take her role as the older sister very seriously today as she told her younger brother Prince Louis off, not once, but twice.
Taking charge, the royal, nine, ordered cheeky Louis to stand up straight on the Buckingham Palace balcony, as the family gathered to watch the flypast at the annual event.
The six-year-old Prince, known for stealing the limelight at large royal events, was stood on the platform in front of thousands of fans and well-wishers with his arms slightly scrunched ahead of the national anthem.
His older sibling then said: 'Louis put your hands down,' according to lipreader Juliet Sullivan, before she tapped his shoulder to get his attention.
Louis then scrunched up his face before placing his hands down by his side and standing up straight to prepare for the national anthem.
Earlier in the day, the prince was once again told off by his sister as she ordered him to 'stop' his dancing, whilst standing at a window in the Horse Guards building, so they could watch King Charles inspecting the troops outside.
Princess Charlotte told Prince Louis to put his hands down before the National anthem played, according to a lip reading expert
As Prince George, Charlotte, and Louis waited by the window with their mother Kate Middleton, 42, the youngest royal started swaying his hips, much to the apparent chagrin of his older sister.
Charlotte, who has been seen issuing instructions to her brothers during previous royal events, appeared to tell her sibling to 'stop dancing'.
However, the young prince appeared unmoved by the command, and continued with his dancing.
Meanwhile, their mother the Princess of Wales appeared to smile while watching the interaction between her children, before she regained her previous, more serious demeanour.
Louis is beloved by many royal fans, thanks to the spirited behaviour he has shown at previous public appearances.
And today has been no difference, with the youngster seen pulling faces as he took a carriage ride with his mother and siblings earlier today.
He appeared less than thrilled during the ride, at point knitting his brows as he stared out of the window, open-mouthed. Another snap of the young prince showed him frowning, with his mouth down-turned.
At another point, the six-year-old appeared bored during the long, formal event, and was seen scrunching his face and playing with curtains, as well as yawning.
Prince Louis scrunched his face after he was told to put his 'hands down' by his big sister Charlotte
The Wales children were pictured looking out of the window of the Horse Guards building, as King Charles inspected the troops below
The children and their mother appeared to be concentrating on watching the activity outside
However, while at the window, Louis started dancing - prompting older sister Charlotte to tell him to stop - which he ignored
The Princess of Wales seemed to smile as she watched the interchange between her two youngest children
Kate Middleton smiles as she watches Princess Charlotte tell Prince Louis to stop dancing
After smiling at her children, the Princess of Wales quickly returned to a more serious demeanor
While the Wales children were accompanied by their mother as they rode to Buckingham Palace in a carriage earlier in the day, their father Prince William took part in the parade on horseback.
And their mother's presence was no doubt reassuring for the youngsters.
The future Queen, who only confirmed that she would be attending the event last night, was the picture of elegance and grace as she donned a crisp white structured pencil dress which had a bow along the neckline. The designer is believed to be Jenny Packham - many of whose creations Kate has donned before.
The garment featured a monochrome belted band which sat on her waist and highlighted her sophisticated frame. Kate opted for a simple black clutch bag and classy pointed white heels, which tied the chic look together.
She wore her glossy brunette tresses in a stylish braided low-bun and completed the look with a coordinating hat by Philip Treacy, and her Cassandra Goad Cavolfiore pearl earrings, which retail for £5,620. She completed the look with her Irish Guards Regimental Brooch - a nod to her role as the regiment's honorary colonel.
Meanwhile, Prince George wore a navy suit with a crisp white shirt and royal blue tie, and a pair of black lace-up shoes. His younger brother Louis wore a navy blazer and shorts, with a white shirt and royal blue tie, and a pair of loafers.
Princess Charlotte wore a navy dress in a sailor-style design, and white strap shoes.
Ahead of her appearance, the Princess penned a moving personal message thanking the public for their support over her cancer diagnosis and revealing she is looking forward to attending the traditional ceremonial day.
Back in March, the Princess disclosed she was undergoing 'preventative chemotherapy' for an undisclosed form of the disease says her treatment is ongoing and will be for several months to come.
Beaming Princess Charlotte couldn't help but smile as she appeared alongside her brother Prince George at today's Trooping the Colour
Kate beams as she accompanies her children in a carriage during today's procession (pictured L-R: Kate, Prince Louis, Princess Charlotte)
Prince Louis (left) pulls a face at the crowd as Princess Charlotte (right) looks regal as they join the parade
Another snap of the popular young Prince showed him grimacing as he rode to Buckingham Palace
Kate arrives with Princess Charlotte and Prince George at Horse Guards Parade today
Prince George, Princess Charlotte, Prince Louis and Kate at Trooping The Colour today
While she will not be returning to public duties full-time, she revealed that she does now feel well enough, however, to undertake a small number of public engagements in the coming months.
To mark the news Kensington Palace have also released a stunning new portrait of the princess taken at Windsor this week by Matt Porteous showing her looking well and in a reflective mood.
In her message the Princess says she has been 'blown away' by all the kind messages of support and encouragement over the last couple of months.
'It really has made the world of difference to William and me and has helped us both through some of the harder times,' she wrote.
Movingly she reveals she is making 'good progress', but adds: As anyone going through chemotherapy will know, there are good days and bad days. On those bad days you feel weak, tired and you have to give in to your body resting. But on the good days, when you feel stronger, you want to make the most of feeling well.'
The Princess of Wales speaks with Prince Louis as they watch Trooping the Colour today
The Princess of Wales leaves Buckingham Palace during Trooping the Colour in London today
The Princess of Wales leaves Buckingham Palace during Trooping the Colour in London today
Prince Louis at Trooping The Colour on Horse Guards Parade in London today
The Princess of Wales leaves Buckingham Palace during Trooping the Colour in London today
She added: 'My treatment is ongoing and will be for a few more months. On the days I feel well enough, it is a joy to engage with school life, spend personal time on the things that give me energy and positivity, as well as starting to do a little work from home.
'I'm looking forward to attending The King's Birthday Parade this weekend with my family and hope to join a few public engagements over the summer, but equally knowing I am not out of the woods yet.
'I am learning how to be patient, especially with uncertainty. Taking each day as it comes, listening to my body, and allowing myself to take this much needed time to heal.
Thank you so much for your continued understanding, and to all of you who have so bravely shared your stories with me.'
The Princess first announced that she had cancer on March 22.
The Princess of Wales leaves Buckingham Palace during Trooping the Colour in London today
The Princess of Wales leaves Buckingham Palace during Trooping the Colour in London today
The Princess of Wales leaves Buckingham Palace during Trooping the Colour in London today
Prince Louis and Princess Charlotte are seen en route to Buckingham Palace in a carriage earlier today
In a hugely moving video statement, recorded at Windsor, she revealed that she had been diagnosed following serious abdominal surgery in January and was already undergoing 'preventative' chemotherapy.
In a deeply personal, unprecedented and emotional video message filmed just two days earlier, Catherine revealed the news had come as a 'huge shock' and that she and William 'have been doing everything we can to process and manage this privately for the sake of our young family'.
'It has taken us time to explain everything to George, Charlotte and Louis in a way that is appropriate for them, and to reassure them that I am going to be ok,' she said.
'As I have said to them; I am well and getting stronger every day by focusing on the things that will help me heal; in my mind, body and spirits. Having William by my side is a great source of comfort and reassurance too. As is the love, support and kindness that has been shown by so many of you. It means so much to us both.'
Kensington Palace said at the time that it would not be sharing details of what kind of cancer the princess has, or what stage of cancer it is and asked people not to speculate.
At the time of her abdominal surgery in January, Kensington Palace said that it was non-cancerous. They confirmed again in March that is the case and that no tests had confirmed the presence of cancer. However post-operative tests subsequently found that cancer 'had been present'.
A week ago, the royal apologised for missing out on a key rehearsal for the event, writing a letter to the Irish Guards, saying: 'I hope I am able to represent you all very soon'.